The Scientific Committee awarded the 3 competition prizes to the following posters:
2006
FUNCTIONAL MONOLITHIC CAPILLARY COLUMNS PREPARED BY RING-OPENING METATHESIS POLYMERIZATION Agnes Mautner1, C. Gatschelhofer2, M. R. Buchmeiser3, T. R. Pieber1,2, F. M. Sinner2* 1 Dept. of Internal Medicine, Diabetes and Metabolism, Medical University Graz, Graz, Austria 2 Inst. of Medical Technologies and Health Management, Joanneum Research, Graz, Austria 3 Leibniz Institute for Surface Modification e. V., Leipzig, Germany Email: frank.sinner@joanneum.at Miniaturization has been one of the main trends in HPLC over the past 10 years. To cope with the increasing requirementsin the field of proteomics and drug discovery, separation media with smaller inner diameters, higher separation efficiency and better sensitivity are needed. Monolithic stationary phases represent an attractive approach for the fabrication of capillary columns, due to their ease of preparation without the requirement of sophisticated packing procedures or the manufacturing of end frits. In recent years, there has been considerable interest in developing monolithic columns bearing functional groups. Ring-Opening Metathesis Polymerization (ROMP) offers the unique possibility to prepare functionalized monolithic columns by applying an in-situ derivatization process. Norborn-2-ene based monolithic capillary columns were prepared from silanized fused-silica capillaries of 200 im inner diameter by ROMP. The still active initiator sites, located at the surface of the structure-forming microglobules, are used for grafting functional groups on the monolithic support. In this study we will report a detailed evaluation at the monolithic support in terms of reproducibility of synthesis and separation efficiency. Further more the preparation of monolithic capillary columns bearing weak cation-exchange groups will be shown.
USE OF MICROARRAY APPROACH FOR QUANTITATIVE DETECTION OF VIRUS LIKE PARTICLES Irina Kalashnikova, Marina Slabospitskaya, Natalia Ivanova, Tatiana Tennikova Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia Email: tennikova@mail.ru Recently suggested glycidyl methacrylate-co–ethylene glycol dimehacrylate (GMA-EDMA) monolithic material as a potential solid matrix for the development of new type of 3-D protein microarrays (biochips), has been investigated for detecting of large-size protein-bearing particles. The results obtained for these virus-mimicking supramolecular structures can be further used for the construction of rapid, high sensitive and high specific test intended for precise diagnosis of some virus respiratory tract infections. Two model affinity pairs transthyretin (thyroxine transport protein, TTR)-monoclonal antibodies (MKAT) to TTR and trypsin–soya bean trypsin inhibitor (TR-SBTI) have been chosen for present investigation. Large-size particles were developed by means of TTR and TR covalent binding to the outer carboxylated surface of specially synthesized and characterized styrene-divinyl benzene latexes. Antibodies and SBTI were immobilized on the surface of prepared by photo-initiated polymerization GMA-EDMA layers via direct reaction of protein’s amino groups with original epoxy grops of polymer support. The detection of obtained biocomplementary complexes between latex-TTR (TR) – MKAT
3.4 µL AND 34 µL CIM MINI DISK MONOLITHIC COLUMNS Jana Vidić, A. Podgornik, A. Štrancar BIA Separations d. o. o., Teslova 30, SI – 1000 Ljubljana, Slovenia E-mail: jana.vidic@monoliths.com Commercially available CIM® disk monolithic columns are intended for very fast analyzes and laboratory purification. Their shape is a compromise to achieve acceptable resolution and binding capacity what make them suitable for wide range of laboratory applications. Separations of complex protein mixtures can be carried out within just a few seconds because of flow unaffected resolution and, on the other hand, purification can be effectuated with high productivity due to flow-unaffected dynamic binding capacity. However, in many cases in the field of molecular biology, only a limited amount of sample is available. In such a case it is beneficial to work with small columns having high resolution or they can be used as affinity columns or bioreactors saving significant amount of valuable ligand. Having this goal in mind we developed CIM® disks with the volume of 1/10th and 1/100th of original volume. In comparison to conventional CIM® disks, they exhibit resolution higher and lower limit of detection, therefore smaller concentrations of target macromolecules can be detected. The separation capability and the protein capacity were tested on anion and cation exchange 3.4 µL and 34 µL mini disk monolithic columns. |
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